sphero ultra rainbow fluorescent particles

ultra rainbow fluorescent particles | Sphero

Description

This product incorporates a single inhabitants of Rainbow Particles that are dyed to a single fluorescent depth. Every Rainbow Particle incorporates a mixture of fluorophores that are stably embedded contained in the particle and could also be excited at any wavelength from 365-650 nm, allowing most channels in a stream cytometer to be calibrated using the an identical set of particles. The Rainbow Particles have emission spectra acceptable with many frequent fluorophores used for immunofluorescent staining by stream cytometric analysis.  Rainbow Fluorescent Particles are supplied in a dropper bottle for helpful shelling out and storage.

Description 

Extraordinarily Rainbow Fluorescent Particles (by Spherotech™) are internally labeled with quite a lot of fluorophores. They exhibit full spectrum fluorescence, along with enhanced UV and Far Crimson fluorescence. The mixture of fluorophores permits Extraordinarily Rainbow particles to be excited at any wavelength from 365 to 650nm. These particles may be present in two diameters, ~3.8µm and ~10.2µm.

Traits
Indicate Diameter: ~3.8µm (3.61-3.99µm) or ~10.2µm (8.1 – 12.0µm);
see your Certificates of Analysis for exact dimension.
Focus: ~2 x 106 particles/mL
Storage Buffer: Deionized water with 0.02% sodium azide and 0.01% NP-4O

Course of
A dilution of 1-2 drops to 1mL will current an sufficient number of particles for stream cytometry use.

Notes
Shake vigorously or vortex briefly sooner than use. Inclusion of a small amount of detergent throughout the diluent will help improve the number of singlets. Diluted particles could also be saved throughout the fridge for future use.
Storage and Stability
Retailer at 2-8˚C. Freezing particles would possibly result in irreversible aggregation and lack of binding train. The product is anticipated to be regular for 12 months from the date of purchase, supplied that it is handled in accordance with
the producer’s options. The reagent must be saved in its opaque bottle.

Safety 

This particle suspension incorporates sodium azide. Sodium azide would possibly react with lead and copper plumbing to sort explosive metallic azides. Upon disposal of material, flush with an enormous amount of water to forestall azide accumulation.
Please search the recommendation of the Supplies Safety Data Sheet for additional information.

 

sphero ultra rainbow fluorescent particles
sphero extremely rainbow fluorescent particles
Sphero™
Aqueous reply containing 0.01%NP40 and ≤ 0.02% sodium azide.
RUO
AB_2869066
Circulation cytometry (Examined All through Enchancment).
SPHERO Rainbow Fluorescent Particles
– A single inhabitants of Rainbow Fluorescent Particles for the alignment of FITC, PE, PE-TR, and PE-Cy5 channels
Catalog No.
Description
Focus
Nominal Dimension
Bundle Dimension
Worth
RFP-20-5
Rainbow Fluorescent Particles (Depth identical to brightest peak in RCP-20-5)
107/mL
1.8-2.2 µm
5 mL
$245.00
RFP-30-5 (1 peak)
Rainbow Fluorescent Particles (Depth identical to brightest peak in RCP-30-5)
107/mL
3.0-3.5 µm
5 mL
$255.00
RFP-30-5A (1 peak)
Rainbow Fluorescent Particles (Depth identical to mid differ FL1 fluorescence in RCP-30-5)
107/mL
3.0-3.4 µm
5 mL
$255.00
RFP-35-5
Rainbow Fluorescent Particles (Depth identical to brightest peak in RCP-35-5)
107/mL
3.5-4.Zero µm
5 mL
$255.00
RFP-50-5
Rainbow Fluorescent Particles
107/mL
5.0-5.9 µm
5 mL
$265.00
RFP-60-5
Rainbow Fluorescent Particles (Depth identical to brightest peak in RCP-60-5) (Useful for FL1, FL2, and FL3 channels solely)
107/mL
6.0-6.4 µm
5 mL
$265.00
RFP-70-5
Rainbow Fluorescent Particles
107/mL
6.5-8.Zero µm
5 mL
$265.00
RFP-100-2
Rainbow Fluorescent Particles
107/mL
8.1-12.Zero µm
2 mL
$210.00

Useful Assay Procedures

This particle mixture (~10×10^6 particles/mL) is useful for routine calibration of stream cytometers. Sooner than use, resuspend the particles by vortexing. Dilution of 3-5 drops of particles to 1 ml of sheath fluid will current an sufficient number of particles for stream cytometric analysis.

Product Notices

  1. Warning: Sodium azide yields extraordinarily toxic hydrazoic acid beneath acidic circumstances. Dilute azide compounds in working water sooner than discarding to steer clear of accumulation of in all probability explosive deposits in plumbing.
  2. Cy is a trademark of GE Healthcare.

 

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