Single-run reversed-phase HPLC method for determining sertraline content, enantiomeric purity, and related substances in drug substance and finished product

• Zachary Long

A direct enantio-, diastereo-, and chemo-selective high-performance liquid chromatographic methodology was developed for figuring out the content material, enantiomeric purity, and associated substances of the chiral antidepressant drug sertraline HCl in a single chromatographic run. The separation was achieved on a chiral stationary part based mostly on amylose tris(3-chloro-5-methylphenylcarbamate) beneath reversed-phase circumstances. The strategy was…