Tag: culture cells in pure serum
Lyophilization and homogenization of biological samples improves reproducibility and reduces standard deviation in molecular biology techniques
Lyophilization is a cheap methodology for organic specimen preservation however detailed tissue-specific reference protocols are nonetheless missing. Furthermore, knowledge are restricted on the long-term stability of proteins and nucleic acids in lyophilized samples. Right here, we provide lyophilization protocols for numerous rat and mouse tissues (kidney, coronary heart, liver, lung, aorta, and pores and skin)…
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Influence of polyethylene terephthalate/ polyamide (PET/PA) bilayer structure on physical and strength-related properties of superpressure angioplasty balloons
Background: Percutaneous transluminal coronary angioplasty (PTCA), together with balloon angioplasty, is a regular scientific invasive therapy for coronary artery illness. The coronary lesion with calcification is tough to dilate and the prevention of balloon failure is particularly vital. Goal: A novel superpressure balloon was fabricated with bilayered construction of polyethylene terephthalate (PET) and PA12 (polyamide).…
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Single-run reversed-phase HPLC method for determining sertraline content, enantiomeric purity, and related substances in drug substance and finished product
A direct enantio-, diastereo-, and chemo-selective high-performance liquid chromatographic methodology was developed for figuring out the content material, enantiomeric purity, and associated substances of the chiral antidepressant drug sertraline HCl in a single chromatographic run. The separation was achieved on a chiral stationary part based mostly on amylose tris(3-chloro-5-methylphenylcarbamate) beneath reversed-phase circumstances. The strategy was…
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