sphero accucount

AccuCount Particles | SPHEROTM

The SPHEROTM AccuCount Particles are designed to be used as reference particles with recognized number of particles per mL for counting completely the cell amount by motion cytometry. The SPHEROTM AccuCount Particles are very easy to utilize and are value environment friendly. The AccuCount Fluorescent Particles are fluorescent in FITC, PE and PE-Cy5 channel. Every…

Read More
micchem

Phylogenetic study of Theileria ovis and Theileria lestoquardi in sheep from Egypt: Molecular evidence and genetic characterization

Background and intention: Ovine theileriosis attributable to Theileria ovis and Theileria lestoquardi is a vital infectious illness affecting small ruminants in areas of the tropic and subtropic zones. There may be restricted research about ovine theileriosis in Egypt; so the current research goals to evaluate the incidence of ovine theileriosis in Egypt on the molecular stage. Supplies and strategies:  Blood…

Read More
micchem

Gallid Alphaherpesvirus 2 in the Egyptian Turkeys: Molecular Characterization and Establishment of a Universal System for Phylogenetic Classification

Introduction: Gallid alphaherpesvirus 2 (GaHV-2) is a extremely contagious oncogenic virus that causes Marek’s illness in chickens and sometimes in turkeys. Amongst 100 genes recognized in GaHV-2 genome, the Meq gene seems to contain viral virulence, oncogenicity, and genetic range. Regardless of the usage of Meq gene sequences in phylogenetic classification of GaHV-2 strains circulating in…

Read More
micchem

β-Defensins from common goby (Pomatoschistus microps) and silver trevally (Pseudocaranx georgianus): Molecular characterization and phylogenetic analysis

Antimicrobial peptides (AMPs) are biologically lively molecules concerned in host protection current in a wide range of organisms. They’re an integral part of innate immunity, forming a entrance line of protection in opposition to potential pathogens, together with antibiotic-resistant ones. Fishes are confirmed to be a potential supply of AMPs as they’re consistently being challenged…

Read More
micchem

Cyperus prophyllatus: An endangered aquatic new species of Cyperus L. (Cyperaceae) with a exceptional spikelet disarticulation pattern among about 950 species, including molecular phylogenetic, anatomical and (micro)morphological data

Cyperus prophyllatus, an endangered new species of Cyperus (Cyperaceae) from an aquatic ecosystem of the Atlantic Forest, Espírito Santo State, southeastern Brazil, is described and illustrated. The spikelet morphology of Cyperus prophyllatus is exclusive among the many c. 950 species of Cyperus in having each a conspicuous spikelet prophyll and a corky rachilla articulation, which…

Read More
micchem

An overview of molecular biology and nanotechnology based analytical methods for the detection of SARS-CoV-2: promising biotools for the rapid diagnosis of COVID-19

At the moment, the 2019 novel coronavirus (2019-nCoV) is drastically affecting 214 nations, inflicting extreme pneumonia in sufferers, which has resulted in lockdown being applied in a number of nations to cease its native transmission. Contemplating this, the speedy screening and correct detection of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2; 2019-nCoV) play a necessary…

Read More
micchem

KRAS G12C-mutant non-small-cell lung cancer: biology, developmental therapeutics, and molecular testing

KRAS mutation is the most typical oncogenic driver in superior non-small cell lung most cancers (NSCLC), occurring in roughly 30% of lung adenocarcinomas. Over 80% of oncogenic KRAS mutations happen at codon 12 the place the glycine residue is substituted by completely different amino acids, resulting in genomic heterogeneity of KRAS-mutant tumors. The KRAS glycine-to-cysteine…

Read More
micchem

Understanding the molecular evolution of tiger diversity through DNA barcoding marker ND4 and NADH dehydrogenase complex using computational biology

Background: Presently, Tigers (the highest predator of an ecosystem) are on the listing of endangered species. Thus the necessity is to know the tiger’s inhabitants genomics to design their conservation methods. Goal: We analyzed the molecular evolution of tiger range utilizing NADH dehydrogenase subunit 4 (ND4), a big electron transport chain part. Strategies: We now have analyzed nucleotide…

Read More
micchem

Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline

Streptomyces rimosus is used for manufacturing of the broad-spectrum antibiotic oxytetracycline (OTC). S. rimosus belongs to Actinomyces species, a big group of microorganisms that produce various set of pure metabolites of excessive significance in lots of elements of our life. On this chapter, we describe particular molecular biology strategies and a classical homologous recombination method…

Read More
micchem

Lyophilization and homogenization of biological samples improves reproducibility and reduces standard deviation in molecular biology techniques

Lyophilization is a cheap methodology for organic specimen preservation however detailed tissue-specific reference protocols are nonetheless missing. Furthermore, knowledge are restricted on the long-term stability of proteins and nucleic acids in lyophilized samples. Right here, we provide lyophilization protocols for numerous rat and mouse tissues (kidney, coronary heart, liver, lung, aorta, and pores and skin)…

Read More