Category: Devices
AccuCount Particles | SPHEROTM
The SPHEROTM AccuCount Particles are designed to be used as reference particles with recognized number of particles per mL for counting completely the cell amount by motion cytometry. The SPHEROTM AccuCount Particles are very easy to utilize and are value environment friendly. The AccuCount Fluorescent Particles are fluorescent in FITC, PE and PE-Cy5 channel. Every…
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ultra rainbow fluorescent particles | Sphero
Description This product incorporates a single inhabitants of Rainbow Particles that are dyed to a single fluorescent depth. Every Rainbow Particle incorporates a mixture of fluorophores that are stably embedded contained in the particle and could also be excited at any wavelength from 365-650 nm, allowing most channels in a stream cytometer to be calibrated…
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Methylflash methylated dna quantification kit
DNA Methylation Quantification DNA methylation performs a necessary place in common organismal enchancment and in cellular differentiation in larger organisms. Gene expression, along with the occasion of virtually all types of most cancers, are moreover tied to DNA methylation. For example, worldwide decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going attributable to methyl-deficiency as…
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Western Blot Stripping Buffer
Course of Warmth the buffer to 50°C Add the buffer to a small plastic area which has a good lid; use a amount which will cowl the membrane Add the membrane. Incubate at 50°C for as a lot as 45 min with some agitation Eradicate the reply as required for ß-mercaptoethanol based totally buffers Rinse the…
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Phylogenetic study of Theileria ovis and Theileria lestoquardi in sheep from Egypt: Molecular evidence and genetic characterization
Background and intention: Ovine theileriosis attributable to Theileria ovis and Theileria lestoquardi is a vital infectious illness affecting small ruminants in areas of the tropic and subtropic zones. There may be restricted research about ovine theileriosis in Egypt; so the current research goals to evaluate the incidence of ovine theileriosis in Egypt on the molecular stage. Supplies and strategies: Blood…
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Gallid Alphaherpesvirus 2 in the Egyptian Turkeys: Molecular Characterization and Establishment of a Universal System for Phylogenetic Classification
Introduction: Gallid alphaherpesvirus 2 (GaHV-2) is a extremely contagious oncogenic virus that causes Marek’s illness in chickens and sometimes in turkeys. Amongst 100 genes recognized in GaHV-2 genome, the Meq gene seems to contain viral virulence, oncogenicity, and genetic range. Regardless of the usage of Meq gene sequences in phylogenetic classification of GaHV-2 strains circulating in…
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β-Defensins from common goby (Pomatoschistus microps) and silver trevally (Pseudocaranx georgianus): Molecular characterization and phylogenetic analysis
Antimicrobial peptides (AMPs) are biologically lively molecules concerned in host protection current in a wide range of organisms. They’re an integral part of innate immunity, forming a entrance line of protection in opposition to potential pathogens, together with antibiotic-resistant ones. Fishes are confirmed to be a potential supply of AMPs as they’re consistently being challenged…
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Understanding the molecular evolution of tiger diversity through DNA barcoding marker ND4 and NADH dehydrogenase complex using computational biology
Background: Presently, Tigers (the highest predator of an ecosystem) are on the listing of endangered species. Thus the necessity is to know the tiger’s inhabitants genomics to design their conservation methods. Goal: We analyzed the molecular evolution of tiger range utilizing NADH dehydrogenase subunit 4 (ND4), a big electron transport chain part. Strategies: We now have analyzed nucleotide…
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Peak force tapping atomic force microscopy for advancing cell and molecular biology
The appearance of atomic pressure microscopy (AFM) supplies an thrilling device to detect molecular and mobile behaviors below aqueous circumstances. AFM is ready to not solely visualize the floor topography of the specimens, but in addition can quantify the mechanical properties of the specimens by pressure spectroscopy assay. However, integrating AFM topographic imaging with pressure…
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Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline
Streptomyces rimosus is used for manufacturing of the broad-spectrum antibiotic oxytetracycline (OTC). S. rimosus belongs to Actinomyces species, a big group of microorganisms that produce various set of pure metabolites of excessive significance in lots of elements of our life. On this chapter, we describe particular molecular biology strategies and a classical homologous recombination method…
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