Biovision Rnase-R

Biovision rnase-r


• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package deal (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)


• For digestion of full RNA, longer incubations of 2-Three hours are generally required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement additional enzyme partway (e.g. 0.5 μl after 1 hour) by way of the method. The higher temperature is particularly useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this may end in non-enzymatic RNA degradation.

• RNase R shows low train on tRNA, rRNA and totally different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use additional RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Keep in mind that spherical RNAs symbolize a small proportion of full RNA (generally 0.1%-0.01%), because of this reality RNase R treatment will most actually finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that purpose, a starting amount of not lower than 10 µg of full RNA is useful for a lot of downstream features.

• Whereas the enzyme could also be heat inactivated the method should not be useful since extreme heat can lead to RNA hurt. Phenol-chloroform precipitation could be utilized instead. For NGS, robust half reversible immobilization (SPRI) bead cleanup is useful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is present, compensate by together with MgCl2 to 1.Zero Mm final focus.


RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by typical splicing, along with circRNAs which come up by way of back-splicing.

• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying varied splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Stay away from repeated freeze-thaw cycles of all components to retain most effectivity. All components
are regular for one 12 months from the date of transport when saved and handled appropriately.


Biovision Rnase-R
Biovision Rnase-R


Cat # +Dimension M1228-500
Dimension 500 U
Highlights RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions -20°C
Supply Conditions Gel Pack
USAGE For Evaluation Use Solely! Not For Use in Individuals.



RNase A

RN103-001 300mg
EUR 2615

RNase A Solution

G118 1.0 ml; 10 mg/ml
EUR 102

anti-RNase L

YF-PA24584 50 ul
EUR 334
Description: Mouse polyclonal to RNase L

anti-RNase H1

YF-PA26995 50 ul
EUR 334
Description: Mouse polyclonal to RNase H1

Thermostable RNAse H

EUR 321

RNase III, E.coli

EUR 224

Murine Rnase inhibitor

R301-01 2000 U
EUR 116

Murine Rnase inhibitor

R301-02 10000 U
EUR 166

Murine Rnase inhibitor

R301-03 20000 U
EUR 219

Rnase A, Pancreatic

abx082443-25mg 25 mg
EUR 189

DNase (RNase-free)

abx098138-1500U 1500 U
EUR 453

RNase-Free water

abx098145-25ml 25 ml
EUR 175

Rnase (90–105)

5-01885 4 x 1mg Ask for price

RNase A (MBG)

409-050 50 mg
EUR 73

RNase A (MBG)

409-250 250 mg
EUR 193

RNase T2 Antibody

abx237324-100ug 100 ug
EUR 509

RNase 1 Antibody

  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

RNase 13 Antibody

  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

Rnase A, 1g

EUR 822

Rnase A, 250mg

EUR 281

RiboSafe RNase Inhibitor

BIO-65027 2500 Units Ask for price

RiboSafe RNase Inhibitor

BIO-65028 10,000 Units Ask for price

RNAse H2A Antibody

24819-100ul 100ul
EUR 390